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Part:BBa_S03639:Design

Designed by Karmella Haynes   Group: iGEM2006_Davidson   (2006-10-30)

From partsregistry.org


(-1,-2) HixC-pBadrev-HixC : TetB-RBSrev-HixC

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 95
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 539


Design Notes

One of the eight permutations of two adjacent hixC-flanked "flippable" parts: pBad and RBS-Tet. This permutation has a reversed pBad promoter followed by a reversed RBS-Tet coding region.

Source

References