Promoter (RhlR & C4-HSL regulated)
Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer.
This is the natural sequence taken from Pseudomonas aeruginosa.
Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
Usage and Biology
RhlR binds to the promoter region and activates transcription when in the presence of its autoinducer -- N-butyryl-homoserine lactone (also known as C4-HSL), which is produced by RhlI.
From [Medina03]: <i>... RhlR binds to this DNA sequence, both in vivo and in vitro, in the presence and in the absence of C4-HSL. The binding of RhlR(C4-HSL) to the las box is a necessary condition for rhlAB expression while the binding of RhlR alone to the same DNA sequence seems to repress rhlAB transcription.</i>
From [Medina03]: <i>The LasR(3-O-C12-HSL) complex can activate rhlAB promoter expression but at a considerably lower level than the RhlR(C4-HSL) complex.</i>
From the data in [Medina03], LasR activates rhlAB at 7.8% of the activation level achieved by RhlR.
From the data in [Pearson97], the activation by LasR is about 3% of that by RhlR.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC