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Regulatory
p(tetR)

Part:BBa_R0040:Design

Designed by June Rhee, Connie Tao, Ty Thomson, Louis Waldman   Group: Registry

From partsregistry.org

TetR repressible promoter

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1


Design Notes

BBa_R0040 (TetR repressible promoter) is based on a cI promoter. It has been modified to include two TetR binding sites.

Constructed by DNA synthesis.

Source

See [1].

References

  1. Lutz R and Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res 1997 Mar 15; 25(6) 1203-10. pmid:9092630. PubMed HubMed [Lutz-1997]
All Medline abstracts: PubMed HubMed
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