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ccdB

Part:BBa_P1016:Design

Designed by Reshma Shetty   Group: Knight Lab   (2007-01-29)

From partsregistry.org

ccdB cassette without ccdA-

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Design Notes

BBa_P1016 differs from BBa_P1010 in that the DNA encoding the mutated antitoxin CcdA protein (ccdA-) has been deleted since it is extraneous to the function of this part as a positive selection marker.

BBa_P1016 differs from BBa_P1011 in that the mutations introduced in BBa_P1011 to remove restriction sites are not present in this part (thus the coding region of ccdB is similar to commercially available versions of ccdB). It does have the "normal" ccd promoter and ccdB coding region. It also has a double TAA stop codon as per BioBrick conventions.

Source

This part was designed based on BBa_P1010 but it lacks ccdA-. It was synthesized via direct synthesis by Codon Devices, Inc. in Cambridge, MA as a part of a larger part BBa_I51020.

References

  1. Bernard P and Couturier M. Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes. J Mol Biol 1992 Aug 5; 226(3) 735-45. pmid:1324324. PubMed HubMed [Bernard-J-Mol-Biol-1992]
  2. Miki T, Park JA, Nagao K, Murayama N, and Horiuchi T. Control of segregation of chromosomal DNA by sex factor F in Escherichia coli. Mutants of DNA gyrase subunit A suppress letD (ccdB) product growth inhibition. J Mol Biol 1992 May 5; 225(1) 39-52. pmid:1316444. PubMed HubMed [Miki-J-Mol-Biol-1992]
  3. Bernard P, Gabant P, Bahassi EM, and Couturier M. Positive-selection vectors using the F plasmid ccdB killer gene. Gene 1994 Oct 11; 148(1) 71-4. pmid:7926841. PubMed HubMed [Bernard-Gene-1994]
  4. Bernard P. New ccdB positive-selection cloning vectors with kanamycin or chloramphenicol selectable markers. Gene 1995 Aug 30; 162(1) 159-60. pmid:7557407. PubMed HubMed [Bernard-Gene-1995]
  5. Bernard P. Positive selection of recombinant DNA by CcdB. Biotechniques 1996 Aug; 21(2) 320-3. pmid:8862819. PubMed HubMed [Bernard-Biotechniques-1996]
All Medline abstracts: PubMed HubMed
  • US Patent Number 5,910,438 "Cloning and/or sequencing vector" Bernard P, Gabant P, University Libre de Bruxelles, 1999. Google Patents
  • US Patent Number 6,180,407 B1 "Cloning and/or sequencing vector" Bernard P, Gabant P, Universit Libre de Bruxelles, 2001. Google Patents
  • US Patent Number 7,176,029 B2 "Cloning and/or sequencing vector" Bernard P, Gabant P, Universit Libre de Bruxelles, 2007. Google Patents
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