Generator
ccdB
Part:BBa_P1011:Design
Designed by Reshma Shetty Group: BE.109 Instructors (2006-03-13)
From partsregistry.org
ccdB cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
Design Notes
This part is derived from BBa_P1010.
The following changes have been made.
- ccdA- has been deleted since its presence is not necessary for ccdB's function as a toxin.
- Some sequence from the 5' end of BBa_P1010 has also been deleted in order to eliminate a BamHI site.
- Silent mutations have been introduced in order to eliminate useful restriction sites and rare codons.
Source
References
- Bernard P, Gabant P, Bahassi EM, Couturier M, Université Libre de Bruxelles. Positive-selection vectors using the F plasmid ccdB killer gene." Gene 1994 Oct 11;148(1):71-4. pmid:7926841. Pubmed
- Bernard P, Gabant P, Université Libre de Bruxelles. Cloning and/or sequencing vector US Patent Number 5,910,438, 1999. Google Patents
- Bernard P, Gabant P, Université Libre de Bruxelles. Cloning and/or sequencing vector US Patent Number 6,180,407 B1, 2001. Google Patents
- Bernard P, Gabant P, Université Libre de Bruxelles. Cloning and/or sequencing vector US Patent Number 7,176,029 B2, 2007. Google Patents
- Bahassi, et al., Université Libre de Bruxelles. Interactions of CcdB with DNA gyrase. Inactivation of Gyra, poisoning of the gyrase-DNA complex, and the antidote action of CcdA., J. Biol. Chem. 1999 274: 10936-10944. pmid:10196173. Pubmed
