Generator
ccdB
Part:BBa_P1010:Design
Designed by Leon Chan Group: Endy Lab, Registry (2004-07-28)
From partsregistry.org
ccdB cell death gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 664
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
ccdB
CcdB, a natural analogue of the quinolone antibiotics (e.g., ciprofloxacin, enoxacin, etc.), binds to the DNA gyrase subunit A, the product of the gyrA gene, turning it into a cellular poison. E.coli strains that carry the gyrA462 mutation (e.g., DB3.1) are resistant to CcdB poisoning.
