Coding

Part:BBa_K726016

Designed by: Verna Huang and Helena Viets   Group: iGEM12_UCSF   (2012-10-02)

T7Prom+HisTag+VioABE

The first three genes in the violacein operon, under the control of a T7 promoter with a lac operator and ribosome binding site.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1147
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 123
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3929
    Illegal AgeI site found at 4125
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

A device was constructed that contained the three enzymes VioA, VioB, and VioE.

As can be seen in the images below, this three enzyme operon was sufficient to produce a green pigment (prodeoxyviolacein) from tryptophan present in the culture media. Samples were grown in LB media with or without IPTG .





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Parameters
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