Regulatory
pBad Weak
Part:BBa_K427000
Designed by Tatiana J. Núñez Elizondo Group: Guests (2010-10-19)
From partsregistry.org
PBad Weak Reverse
PBad Weak Rev is the reverse complement of the Arabinose inducible promoter created by British Columbia in 2009. It is a mutation of the wild type PBad promoter that increases the amount of arabinose needed to induce the transcription of the downstream sequences.
Usage and Biology
It can be used to promote the transcription of DNA sequences coded in the antisense strand of DNA it can also be used to build a jammer device in which the translation of a protein is stopped because of the mRNA produced by the sense and antisense strand are complimentary.
This part has no special safety considerations.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 61
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
References
BBa_K206001 part created by British Columbia team in 2009.
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