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Plasmid_Backbone

Part:BBa_K359201

Designed by George Zarubin   Group: iGEM10_Waterloo   (2010-07-28)

From partsregistry.org


pSB3K3-S-rbsRFP-P

Inspired by the measkit BBa_I20260. The RFP measkit template with swappable promoter part, taken from BBa_J61002.

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Illegal EcoRI site found at 3598
    Illegal NheI site found at 2253
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal NotI site found at 878
    Illegal NotI site found at 3604
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3598
    Illegal XhoI site found at 1046
    Illegal XhoI site found at 1889
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3598
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3598
    Illegal XbaI site found at 3613
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal AgeI site found at 581
    Illegal AgeI site found at 693
    Illegal AgeI site found at 2339
    Illegal AgeI site found at 2662


Retrieved from "http://partsregistry.org/Part:BBa_K359201"
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