pbad CDEG plambda AB
This part contains two operons. The first consists of the LuxCDEG genes from V.fischeri that have been codon optimised for use in E.coli. These genes produce the substrate for the LuxAB genes encoded by the second reading frame. The LuxAB genes have been derived from Xenorhabdus luminescens. The first operon is under control of the arabinose induced pBAD promoter, the second is controlled by the strong constitutive pLambda promoter.
As the proteins of the V.fischeri Lux operon are easily damaged by a high incubation temperature, transformed cells should not be grown at more than 30°C if light production is desired.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1205
- 21Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 979
Illegal AgeI site found at 4322
Illegal AgeI site found at 4537
Illegal AgeI site found at 5125
Illegal AgeI site found at 6108
- 1000COMPATIBLE WITH RFC