Generator

Part:BBa_K325906

Designed by: Hannah Copley and Anja Hohmann   Group: iGEM10_Cambridge   (2010-10-23)

pbad CDEG plambda AB

This part contains two operons. The first consists of the LuxCDEG genes from V.fischeri that have been codon optimised for use in E.coli. These genes produce the substrate for the LuxAB genes encoded by the second reading frame. The LuxAB genes have been derived from Xenorhabdus luminescens. The first operon is under control of the arabinose induced pBAD promoter, the second is controlled by the strong constitutive pLambda promoter.

As the proteins of the V.fischeri Lux operon are easily damaged by a high incubation temperature, transformed cells should not be grown at more than 30°C if light production is desired.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 4322
    Illegal AgeI site found at 4537
    Illegal AgeI site found at 5125
    Illegal AgeI site found at 6108
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
outputLight
positive_regulatorsL-arabinose