Generator

Part:BBa_K325905

Designed by: Will Handley and Emily Knott   Group: iGEM10_Cambridge   (2010-10-23)

Bacterial Lux reporter
CDEG pλ AB
(E. coli optimised)

This part contains two operons. The first consists of the LuxCDEG genes from V.fischeri that have been codon optimised for use in E.coli. These genes produce the substrate for the LuxAB genes encoded by the second reading frame. The LuxAB genes have been derived from Xenorhabdus luminescens. The first operon can be placed under any promoter to achieve inducible or constitutive light output. The second operon is controlled by the strong constitutive pLambda promoter.

As the proteins of the V.fischeri Lux operon are easily damaged by a high incubation temperature, transformed cells should not be grown at more than 30°C if light production is desired.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3112
    Illegal AgeI site found at 3327
    Illegal AgeI site found at 3915
    Illegal AgeI site found at 4898
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/reporter/light
Parameters
None