Generator

Part:BBa_K325903

Designed by: Peter Emmrich and Anja Hohmann   Group: iGEM10_Cambridge   (2010-10-23)

luxEG
V. Fischeri
(E. coli optimised)

This part contains the Genes LuxE and LuxG from V.fischeri that have been codon optimised for use in E.coli. These genes are involved in the production of the substrate for the bacterial luciferase encoded by the LuxA and LuxB genes. As the proteins of the V.fischeri Lux operon are easily damaged by a high incubation temperature, transformed cells should not be grown at more than 30°C if light production is desired.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 714
    Illegal AgeI site found at 929
    Illegal AgeI site found at 1517
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None