Generator

Part:BBa_K325902

Designed by: Theo Sanderson and Bill Collins   Group: iGEM10_Cambridge   (2010-10-23)

luxCD
V. Fischeri
(E. coli optimised)

This part contains the Genes LuxE and LuxG from V.fischeri that have been codon optimised for use in E.coli. These genes are involved in the production of the substrate for the bacterial luciferase encoded by the LuxA and LuxB genes. As the proteins of the V.fischeri Lux operon are easily damaged by a high incubation temperature, transformed cells should not be grown at more than 30°C if light production is desired.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None