Yellow Firefly Luciferase (under pBAD)
(E. coli optimised)
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase. Then a Pro452Ser mutation was used to create a yellow bioluminescence.
Colour of emission
This part is based on Part BBa_K325219. We resynthesised this part to remove three PstI restriction sites, making it compatible with BioBrick assembly standards 10 and 23.
Unfortunately, in the process of mutagenesis to create the colour change a SpeI site was introduced at site 3044. We have ordered primers to remove this site by a substitution from T->C at base 3046. BBa K325259 (planning) will be the successor to this part compatible with Assembly Standards 10 and 23. Unfortunately we did not have time to complete this before the submission deadline, therefore the part will be submitted during November 2010. In the meantime it is possible to use this part with RFC 57.
- 10Illegal SpeI site found at 3044
- 12Illegal NheI site found at 1205
Illegal NheI site found at 3278
Illegal SpeI site found at 3044
- 21Illegal BamHI site found at 1144
Illegal BamHI site found at 2169
- 23Illegal SpeI site found at 3044
- 25Illegal SpeI site found at 3044
Illegal AgeI site found at 979
Illegal AgeI site found at 2118
Illegal AgeI site found at 3820
- 1000Illegal SpeI site found at 3044