Red Firefly Luciferase and LRE
(E. coli optimised)
This part is contains the coding sequence for a mutant light-emitting enzyme (luciferase) and luciferin regenerating enzyme (LRE) from the Japanese firefly, Luciola cruciata. It will make bacteria emit light at a reddish wavelength when placed under a promoter, provided the media is supplemented with luciferin.
The luciferin regenerating enzyme will convert oxyluciferin into CHBT. CHBT is converted to more luciferin in the presence of D-cysteine.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 2060
- 21Illegal BamHI site found at 951
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 900
Illegal AgeI site found at 2602
- 1000COMPATIBLE WITH RFC