Generator

Part:BBa_K325209

Designed by: Anja Hohmann and Will Handley   Group: iGEM10_Cambridge   (2010-09-17)

Green Firefly Luciferase and LRE (under pBAD)
L. Cruciata
(E. coli optimised)

This is the wild type luciferase from Luciola cruciata with a luciferin regenerating enzyme both under the control of an arabinose induced promoter.

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 3044
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 3278
    Illegal SpeI site found at 3044
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2169
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 3044
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3044
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2118
    Illegal AgeI site found at 3820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SpeI site found at 3044


[edit]
Categories
//classic/generator/uncategorized
Parameters
outputLight
positive_regulatorsL-arabinose