Translational_Unit

Part:BBa_K325202

Designed by: Paul Masset and Theo Sanderson   Group: iGEM10_Cambridge   (2010-10-23)

Luciferin Regenerating Enzyme (LRE)
L. Cruciata
(E. coli optimised)


LRE (Luciferin Regenerating Enzyme) is a protein found in the light producing lantern organs of fireflies. It catalyses the conversion of oxyluciferin back into luciferin upon the addition of D-cysteine, relieving inhibition of luciferase by oxyluciferin as well as fueling the luciferin pool of the cell.

The ability of a cell to express LRE will increase both the brightness and glowing time of luciferin-requiring luciferases.

For more information refer to our wiki.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 900
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None