Generator

Part:BBa_K325109

Designed by: Anja Hohmann and Peter Emmrich   Group: iGEM10_Cambridge   (2010-09-15)

EPIC Firefly Luciferase and LRE (under pBAD)
P. Pyralis
(E. coli optimised)

Generates EPIC luciferase and LRE when induced by Arabinose.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3798
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2166
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
outputLight
positive_regulatorsL-arabinose