EPIC Firefly Luciferase (under pBAD)
(E. coli optimised)
This part is a translational unit for a mutant of the luciferase from the North American firefly (P. pyralis). It is under the control of an Arabinose induced promoter (pBAD). D-Luciferin has to be added to obtain light output.
EPIC stands for Enhanced Photon Initiating Complex. It is described in Fujii et al. 2007 as having a 10 times higher substrate affinity and luminescence output compared to wildtype.
The part is codon optimised for expression in E.coli.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1205
- 21Illegal BglII site found at 2845
Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 979
- 1000COMPATIBLE WITH RFC