Generator

Part:BBa_K325108

Designed by: Theo Sanderson and Paul Masset   Group: iGEM10_Cambridge   (2010-10-23)

EPIC Firefly Luciferase (under pBAD)
P. Pyralis
(E. coli optimised)


This part is a translational unit for a mutant of the luciferase from the North American firefly (P. pyralis). It is under the control of an Arabinose induced promoter (pBAD). D-Luciferin has to be added to obtain light output.

EPIC stands for Enhanced Photon Initiating Complex. It is described in Fujii et al. 2007 as having a 10 times higher substrate affinity and luminescence output compared to wildtype.

The part is codon optimised for expression in E.coli.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2845
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
outputLight
positive_regulatorsL-arabinose