Translational_Unit

Part:BBa_K325101

Designed by: Ben Reeve and Hannah Copley   Group: iGEM10_Cambridge   (2010-10-23)

EPIC Firefly Luciferase
Photinus Pyralis
(E. coli optimised)


This part is a translational unit for a mutant of the luciferase from the North American firefly (P. pyralis).

EPIC stands for Enhanced Photon Initiating Complex. It is described in Fujii et al. 2007 as having a 10 times higher substrate affinity and luminescence output compared to wildtype.

The part is codon optimised for expression in E.coli.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1627
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None