Designed by: Ben Reeve and Paul Masset   Group: iGEM10_Cambridge   (2010-09-15)

EPIC Firefly Luciferase and LRE
P. Pyralis
(E. coli optimised)

This part is a translational unit for a mutant of the luciferase from the North American firefly (P. pyralis) as well as this species' luciferin regenerating enzyme (LRE). It is under the control of an Arabinose induced promoter (pBAD). D-Luciferin has to be added to obtain light output.

EPIC stands for Enhanced Photon Initiating Complex. It is described in Fujii et al. 2007 as having a 10 times higher substrate affinity and luminescence output compared to wildtype.

The part is codon optimised for expression in E.coli.

[1]: S.M. Marques and J.C.G. Esteves da Silva, (2009) Firefly Bioluminescence: A Mechanistic Approach of Luciferase Catalyzed Reactions,Life 61, 6-17.

[2]: T. Nakatsu et al. (2006) Structural Basis for the spectral difference in luciferase bioluminescence, Nature 440(16), 372-376.

[3]: K. Gomi and N. Kajiyama, (2001) Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin, The Journal of Biological Chemistry, 276(39), 36508-36513.