Produces a purple pigment (violacein) from L-tryptophane. The operon contains five genes (VioA-E) each with their own ribosome binding sites.
2010 iGEM team Slovenia has further characterised vio operon (Part:BBa_K274002) and vio operon ABCE (Part:BBa_K274004). We have analysed the products of both parts by thinlayer liquid chromatography and mass spectrometry (for a detailed description of the procedures see the 2010 iGEM team Slovenia wiki). For results of our experiments see the page experience.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1029
- 21Illegal BglII site found at 5602
Illegal BamHI site found at 4289
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 3814
Illegal AgeI site found at 4010