Coding
Part:BBa_K194001
Designed by Christian Kaas Group: iGEM09_DTU_Denmark (2009-07-13)
From partsregistry.org
GFP (a yeast- and FACS-optimized GFP variant),
This constitutively expressed GFP, originally from Aequorea victoria was codon optimized and developed as a reporter for gene expression in Saccharomyces cerevisiae according to Cormak et al (1997). In addition, two amino acid changes were included enabling a far more bright florescence compared to the wild type in E.coli. Maximum absorbance: 490 nm
By expanding the host organism, this is an improvement of an existing biobrick: BBa_E0040
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
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