Constitutive pabA expression construct
pabA is one of three genes (pabA, pabB, pabC) involved in the para-aminobenzoic acid (pABA) synthesis pathway from chorismate. In this construct, pabA (para-aminobenzoate synthetase component II) has been placed behind the strong promoter J23100 and the strong RBS B0034, and in front of the terminator B0015. Currently it is in the high copy plasmid pSB1AK3.
Usage and Biology
When measured using the Caltech iGEM PABA HPLC protocol, K137055 in pSB1AK3 in DH10B produced 700ng/mL of PABA (vs. negligible production for DH10B without K137055).
HPLC trace. PABA elutes at 4.8 minutes. Blue: DH10B (negative control), Green: K137055
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC