Composite
Part:BBa_K077557
Designed by Auke van Heel Group: iGEM08_Groningen (2008-10-11)
From partsregistry.org
Part 1 of the Conways game of life system
This part constitutively produces LuxR, which forms a complex with HSL from neighboring cells. These LuxR.HSL complexes bind on the cI+LuxR promoter to produce LuxI, responsible for the HSL production to send signals to its own neighbors. Also GFP will be produced to visualize the ON state.
Input: 3OC6HSL
Output: LuxR, LuxI (producing 3OC6HSL) and GFP
Flow cytometry results of K077557. A total of 20.000 events were measured. Y-axis indicates number of events. X-axis indicates fluorescence on a logarithmic scale. Z-axis indicates time after induction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1766
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
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Categories
Reviews
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Unrated Part
Experience: Works
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4
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3
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2
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1
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