"
DNA Available
Experience: Works
Part:BBa_J61002
Entered: 2006-07-31
Designed by John Anderson Group: Arkin Lab
From partsregistry.org
EX-Ptet-S-rbsRFP-P "RFP reporter"
Plasmid for construction of promoter basic parts and their derivatives. This plasmid can be used two ways:
- Insertion of a promoter element between XbaI and SpeI sites results in a RFP reporter while retaining the ability to do biobrick assembly. Part J61002 is the tet promoter variant of the plasmid.
- Insertion of a protein generating device or RNA gene (cutting the part with XbaI/PstI, inserting into SpeI/PstI of J61002) results in a standard pSB1A2 plasmid containing an r0040.yourpart composite.
See similar part J61003 for a similar method of construction for constructing open reading frame parts, or r0040.b0034.your ORF composite parts in pSB1A2. The same procedure can be used with part J23019 for the same effect in a pSB3C6 plasmid backbone.
- RS 19:58, 24 November 2008 (UTC): The original entry of this part included both a plasmid and plasmid backbone. It has been split into a plasmid backbone and part. The physical Registry distribution has been updated accordingly.
The original part is now BBa_J61002-R0040.
Sequence and Features
EcoRI site found in the sequence at base pair 2927.
XbaI site found in the sequence at base pair 2942.
SpeI site found in the sequence at base pair 2.
PstI site found in the sequence at base pair 885.
NotI site found in the sequence at base pair 878.
NotI site found in the sequence at base pair 2933.
XbaI site found in the sequence at base pair 2942.
SpeI site found in the sequence at base pair 2.
PstI site found in the sequence at base pair 885.
NotI site found in the sequence at base pair 878.
NotI site found in the sequence at base pair 2933.
