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YYC912

Part:BBa_J45999:Experience

Designed by MIT iGEM 2006   Group: iGEM2006_MIT   (2006-10-30)

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Applications of BBa_J45999

Odor-free chassis for odor biosynthetic devices and systems.


User Reviews


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Reshma Shetty

The odor-free chassis (BBa_J45999) has no smell and produces no detectable levels of indole.


Characterization

Gas chromatography analysis of odor-free chassis

Odor-free chassis does not produce the fecal odor indole To verify our selection of E. coli strain YYC912 as an odor-free chassis, we analyzed cultures of each strain for indole production using gas chromatography. E. coli strain YYC912 had no detectable indole (A) whereas E. coli strain TOP10 had high levels of indole (B). The retention time of the indole peak from E. coli strain TOP10 is identical to that of the pure indole standard (C).
Odor-free chassis does not produce the fecal odor indole
To verify our selection of E. coli strain YYC912 as an odor-free chassis, we analyzed cultures of each strain for indole production using gas chromatography. E. coli strain YYC912 had no detectable indole (A) whereas E. coli strain TOP10 had high levels of indole (B). The retention time of the indole peak from E. coli strain TOP10 is identical to that of the pure indole standard (C).


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