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Regulatory
p(osmY)

Part:BBa_J45992

Designed by Stephen Payne   Group: iGEM2006_MIT   (2006-07-11)

From partsregistry.org

Full-length stationary phase osmY promoter

BBa_J45992 is a stationary phase promoter derived from the promoter that controls transcription of osmY in Escherichia coli [1, 2]. BBa_J45992 is active in stationary phase and under high osmotic pressure conditions.

Usage and Biology

Expression of osmY is dependent on σS in vivo [3]. Schellhorn et al. previously demonstrated that an osmY::lacZ fusion generated the highest transcriptional signal in stationary phase as compared to nine other σS-dependent promoter-lacZ fusions [4, 5]. In addition, the osmY::lacZ fusion generated only a small transcriptional signal during exponential growth.

  1. Yim HH and Villarejo M. osmY, a new hyperosmotically inducible gene, encodes a periplasmic protein in Escherichia coli. J Bacteriol 1992 Jun; 174(11) 3637-44. pmid:1317380. PubMed HubMed [Yim-1992]
  2. Yim HH, Brems RL, and Villarejo M. Molecular characterization of the promoter of osmY, an rpoS-dependent gene. J Bacteriol 1994 Jan; 176(1) 100-7. pmid:8282684. PubMed HubMed [Yim-1994]
  3. Hengge-Aronis R, Lange R, Henneberg N, and Fischer D. Osmotic regulation of rpoS-dependent genes in Escherichia coli. J Bacteriol 1993 Jan; 175(1) 259-65. pmid:8416901. PubMed HubMed [Hengge-Aronis-1993]
  4. Schellhorn HE, Audia JP, Wei LI, and Chang L. Identification of conserved, RpoS-dependent stationary-phase genes of Escherichia coli. J Bacteriol 1998 Dec; 180(23) 6283-91. pmid:9829938. PubMed HubMed [Schellhorn-1998]
  5. Vijayakumar SR, Kirchhof MG, Patten CL, and Schellhorn HE. RpoS-regulated genes of Escherichia coli identified by random lacZ fusion mutagenesis. J Bacteriol 2004 Dec; 186(24) 8499-507. doi:10.1128/JB.186.24.8499-8507.2004 pmid:15576800. PubMed HubMed [Vijaykumar-2004]
All Medline abstracts: PubMed HubMed

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]


Retrieved from "http://partsregistry.org/Part:BBa_J45992"
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Categories
//chassis/prokaryote/ecoli
//direction/forward
//iGEM2006/MIT/favorites
//promoter
//regulation/constitutive
//rnap/prokaryote/ecoli/sigmaS
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