Part:BBa_J45700
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Wintergreen odor biosynthetic system
We assembled a complete wintergreen odor biosynthetic system (BBa_J45700) by combining the salicylic acid (BBa_J45320) and wintergreen odor generators (BBa_J45120). BBa_J45700 catalyzes the conversion of the cellular metabolite chorismate to methyl salicylate or wintergreen odor.
Usage and Biology
To construct a complete wintergreen odor biosynthetic system, we needed to design an additional biosynthetic device for wintergreen odor precursor production. We designed a biosynthetic device for production of salicylate from the cellular metabolite chorismate (BBa_J45320) based on the pchBA genes from Pseudomonas aeruginosa. The enzyme PchA, an isochorismate synthase, catalyzes the conversion of chorismate to isochorismate. The enzyme PchB, an isochorismate pyruvate-lyase, catalyzes the conversion of isochorismate to salicylate and pyruvate. Serino et al. previously demonstrated salicylate formation in E. coli using the pchBA genes.
Sequence and Features Content-type: text/html
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