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Stationary phase banana odor biosynthetic system

Design Notes

We recommend use of strain BBa_J45999 as it does not produce indole. Indole is responsible for the foul smell of wt e.coli, and masks the banana scent produced by J45600.

Source

This system is composed of devices J45250 and J45400.

References

ATF1

1. [ATF1]
2. Horton CE, Huang KX, Bennett GN, and Rudolph FB. Heterologous expression of the Saccharomyces cerevisiae alcohol acetyltransferase genes in Clostridium acetobutylicum and Escherichia coli for the production of isoamyl acetate. J Ind Microbiol Biotechnol 2003 Jul; 30(7) 427-32. doi:10.1007/s10295-003-0070-0 pmid:12937998. PubMed HubMed [Horton03]
3. Yoshimoto H, Fukushige T, Yonezawa T, and Sone H. Genetic and physiological analysis of branched-chain alcohols and isoamyl acetate production in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 2002 Aug; 59(4-5) 501-8. doi:10.1007/s00253-002-1041-5 pmid:12172617. PubMed HubMed [BAT2]
4. Yoshimoto H, Fukushige T, Yonezawa T, and Sone H. Genetic and physiological analysis of branched-chain alcohols and isoamyl acetate production in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 2002 Aug; 59(4-5) 501-8. doi:10.1007/s00253-002-1041-5 pmid:12172617. PubMed HubMed [THI3]

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