DNA Available

Experience: Fails

Generator

3-MBG

Part:BBa_J45400:Design

Designed by MIT IGEM 2006 Group: iGEM2006_MIT (2006-08-21)

From partsregistry.org

3-methylbutanal generator

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1250
Illegal EcoRI site found at 2151
Illegal EcoRI site found at 2850
Illegal EcoRI site found at 3036
Illegal SpeI site found at 1944
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1250
Illegal EcoRI site found at 2151
Illegal EcoRI site found at 2850
Illegal EcoRI site found at 3036
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1250
Illegal EcoRI site found at 2151
Illegal EcoRI site found at 2850
Illegal EcoRI site found at 3036
Illegal SpeI site found at 1944
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1250
Illegal EcoRI site found at 2151
Illegal EcoRI site found at 2850
Illegal EcoRI site found at 3036
Illegal SpeI site found at 1944
Illegal AgeI site found at 1114

Design Notes

See biosynthetic pathway from leucine to isoamyl alcohol (the precursor to isoamyl acetate or banana odor)
BBa_J45009 has BioBrick restriction sites EcoRI and SpeI. Thus, BBa_J45400 can only be used as a suffix part.

Source

BBa_R0011 and BBa_J45398.

References

See Part:BBa_J45008:Design#References and Part:BBa_J45009:Design#References.

Retrieved from "http://partsregistry.org/Part:BBa_J45400:Design"