Generator
WOG
Part:BBa_J45100:Design
Designed by Boyuan Zhu Group: iGEM2006_MIT (2006-08-09)
From partsregistry.org
Wintergreen odor enzyme (BSMT1) generator
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Design Notes
BSMT CDS taken from GenBank. The following primers were used for cloning:
Designed Forward Strand (1 and 2):
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG GAA GTT GTT GAA GTT C -3'
- LENGTH: 19
- GC CONTENT: 36.8 %
- MELT TEMP: 47.8 ºC
Designed Reverse Strand (phBSMT1): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAG GAG -3'
- LENGTH: 22
- GC CONTENT: 27.3 %
- MELT TEMP: 46.6 ºC
Source
Species: Petunia X. hybrida from GenBank (AY233465); (also available: A. thaliana and N. suaveolens). We obtained our version of BSMT from N. Dudareva.
References
- Underwood BA, Tieman DM, Shibuya K, Dexter RJ, Loucas HM, Simkin AJ, Sims CA, Schmelz EA, Klee HJ, and Clark DG. Ethylene-regulated floral volatile synthesis in petunia corollas. Plant Physiol 2005 May; 138(1) 255-66. doi:10.1104/pp.104.051144 pmid:15849311.
- Negre F, Kish CM, Boatright J, Underwood B, Shibuya K, Wagner C, Clark DG, and Dudareva N. Regulation of methylbenzoate emission after pollination in snapdragon and petunia flowers. Plant Cell 2003 Dec; 15(12) 2992-3006. doi:10.1105/tpc.016766 pmid:14630969.
