1 Registry Star
Coding
ATF1
Part:BBa_J45014:Design
Designed by André Green II Group: iGEM2006_MIT (2006-07-31)
From partsregistry.org
alcohol acetyltransferase I; converts isoamyl alcohol to isoamyl acetate (banana odor)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 99
Illegal BamHI site found at 1426 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
Design Notes
- Constructed by PCR.
- Eliminated EcoRI site at base pair 412 by changing base pair 414 from A to G
- BBa_J45014 differs from BBa_J45006 by a single point mutation at base pair 1148 (T -> C).
Template
Saccharomyces cerevisiae strain ACLY387 genome
Forward primer
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GAA TGA AAT CGA TGA GAA AAA TC -3'
Reverse primer
5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAa ggg cct aaa agg aga gct ttg -3'
Forward mutagenesis primer
5'- GAA GCA AAT ATT AGA AGA GTT CAA AAA TAG TAA GGG -3'
Reverse mutagenesis primer
5'- CCC TTA CTA TTT TTG AAC TCT TCT AAT ATT TGC TTC -3'
Source
ATF1 gene from Saccharomyces cerevisiae (GenBank Z75285)
References
- Fujii T, Nagasawa N, Iwamatsu A, Bogaki T, Tamai Y, and Hamachi M. Molecular cloning, sequence analysis, and expression of the yeast alcohol acetyltransferase gene. Appl Environ Microbiol 1994 Aug; 60(8) 2786-92. pmid:8085822.
- Horton CE, Huang KX, Bennett GN, and Rudolph FB. Heterologous expression of the Saccharomyces cerevisiae alcohol acetyltransferase genes in Clostridium acetobutylicum and Escherichia coli for the production of isoamyl acetate. J Ind Microbiol Biotechnol 2003 Jul; 30(7) 427-32. doi:10.1007/s10295-003-0070-0 pmid:12937998.
- Singh R, Vadlani PV, Harrison ML, Bennett GN, and San KY. Aerobic production of isoamyl acetate by overexpression of the yeast alcohol acetyl-transferases AFT1 and AFT2 in Escherichia coli and using low-cost fermentation ingredients. Bioprocess Biosyst Eng 2008 Jun; 31(4) 299-306. doi:10.1007/s00449-007-0159-3 pmid:17891501.
