Coding
THI3
Part:BBa_J45009:Design
Designed by Veena Venkatachalam Group: iGEM2006_MIT (2006-06-07)
From partsregistry.org
ketoisocaproate decarboxylase (THI3); converts alpha-ketoisocaproate to 3-methylbutanal
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789
Illegal SpeI site found at 697 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789
Illegal SpeI site found at 697 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789
Illegal SpeI site found at 697
Design Notes
- See biosynthetic pathway from leucine to isoamyl alcohol (the precursor to isoamyl acetate or banana odor)
- Constructed by PCR
Template
Saccharomyces cerevisiae strain ACLY387 genome
Forward primer
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA Gat gaa ttc tag cta tac aca gag -3'
Reverse primer
5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAg tat cca act tga ttt ttt ttt aga ag -3'
Source
- THI3 from Saccharomyces cerevisiae (GenBank Z74128 REGION: 520..2349)
- THI3 SGD entry
References
Refers only indirectly to THI3 [1].
- Dickinson JR, Lanterman MM, Danner DJ, Pearson BM, Sanz P, Harrison SJ, and Hewlins MJ. A 13C nuclear magnetic resonance investigation of the metabolism of leucine to isoamyl alcohol in Saccharomyces cerevisiae. J Biol Chem 1997 Oct 24; 272(43) 26871-8. pmid:9341119.
- Yoshimoto H, Fukushige T, Yonezawa T, and Sone H. Genetic and physiological analysis of branched-chain alcohols and isoamyl acetate production in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 2002 Aug; 59(4-5) 501-8. doi:10.1007/s00253-002-1041-5 pmid:12172617.
- Nishimura H, Kawasaki Y, Kaneko Y, Nosaka K, and Iwashima A. A positive regulatory gene, THI3, is required for thiamine metabolism in Saccharomyces cerevisiae. J Bacteriol 1992 Jul; 174(14) 4701-6. pmid:1624458.
- Lilly M, Bauer FF, Styger G, Lambrechts MG, and Pretorius IS. The effect of increased branched-chain amino acid transaminase activity in yeast on the production of higher alcohols and on the flavour profiles of wine and distillates. FEMS Yeast Res 2006 Aug; 6(5) 726-43. doi:10.1111/j.1567-1364.2006.00057.x pmid:16879424.
