1 Registry Star
Coding
BSMT1
Part:BBa_J45004:Design
Designed by Boyuan Zhu Group: iGEM2006_MIT (2006-06-07)
From partsregistry.org
SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 663
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
Design Notes
Constructed by PCR.
Template
pET-28a-BSMT1[1]
- We thank Natalia Dudareva, Department of Horticulture and Landscape Architecture, Purdue University for the gift of the expression vector with the petunia BSMT1 coding sequence.
Forward primer
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GGA AGT TGT TGA AGT TC -3'
Reverse primer
5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAG GAG -3'
Source
- BSMT1 gene from Petunia x hybrida (GenBank AY233465)
References
- Negre F, Kish CM, Boatright J, Underwood B, Shibuya K, Wagner C, Clark DG, and Dudareva N. Regulation of methylbenzoate emission after pollination in snapdragon and petunia flowers. Plant Cell 2003 Dec; 15(12) 2992-3006. doi:10.1105/tpc.016766 pmid:14630969.
- Underwood BA, Tieman DM, Shibuya K, Dexter RJ, Loucas HM, Simkin AJ, Sims CA, Schmelz EA, Klee HJ, and Clark DG. Ethylene-regulated floral volatile synthesis in petunia corollas. Plant Physiol 2005 May; 138(1) 255-66. doi:10.1104/pp.104.051144 pmid:15849311.
