Coding
Part:BBa_J36843:Design
Designed by Perry Tsai Group: iGEM2006_Harvard (2006-10-29)
From partsregistry.org
Streptavidin, single-chain dimer + His6 tag (no start codon)
Content-type: text/html
Software error:
Can't call method "sequence" without a package or object reference at /web-site/partsregistry.org/cgi/lib/AssemblySystem.pm line 216.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
Design Notes
There was a PstI site CTGCAG at nucleotides 617-622 of the part, which was mutated to CTGCGG.
There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.
Source
PCR off a plasmid from Filiz Aslan at Boston University.
