Coding
Part:BBa_J36841:Design
Designed by Perry Tsai Group: iGEM2006_Harvard (2006-10-29)
From partsregistry.org
Streptavidin, wild-type core + His6 tag (no start codon)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 40
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 82
Illegal AgeI site found at 133
Design Notes
There was originally a XbaI site TCTAGA at nucleotides 118-123 of the part, which was mutated to AGCCGC. It is missing its start codon at the beginning, as the intention was to fuse this with surface display domains upstream.
There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.
Source
PCR off a plasmid obtained from Alice Ting's lab at MIT.
