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Part:BBa_J3107

Designed by Karmella Haynes   Group: iGEM2006_Davidson   (2006-10-12)

From partsregistry.org


AraC-PC, pLac-Hin

AraC-PC (part BBa_I13458) should constitutively express the AraC protein (inhibitor of the pBad promoter). Expression of Hin invertase is under control of the pLac promoter. Therefore, Hin should be expressed in the presence of IPTG. This part was designed for two reasons:

1. To regulate DNA inversion (via controlled Hin invertase expression) on a second plasmid 2. To regulate the transcription of a pBad driven reporter (via the addition of arabinose) on the second plasmid

Sequence and Features Content-type: text/html

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Categories
//function/recombination/hin
Reviews
Unrated Part
Experience: Works
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1
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Twins
BBa_S03609 Deleted
Parameters
o_h
proteinAraC, Hin
rbs147
tagNone