1 Registry Star
Part:BBa_J3101:Design
From partsregistry.org
Recombinational Enhancer (RE) for Hin/Hix inverting
- 10COMPATIBLE WITH RFC[10]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
Design Notes
The C > T point mutation occurred during cloning. It is located in the spacer region between the Fis binding sites; therefore, we believe that the mutation will not impact RE function. There is an insertion immediately before the BioBrick suffix and several bases after the distal fis binding site that should affect RE function. RE is cloned in plasmid pSB1A2.
The BioBrick prefix and suffix on this part are not wildtype but the restriction sites are still viable.
| Standard BioBrick Cloning Sites (Knight) | 5'--GAATTC GCGGCCGC T TCTAGA G ----insert---- T ACTAGT A GCGGCCG CTGCAG-- 3'--CTTAAG CGCCGGCG A AGATCT C -------------- A TGATCA T CGCCGGC GACGTC-- |
| BBa_J31001 Cloning Sites | 5'--GAATTC GCGGCCGC T TCTAGA * ------RE------ G ACTAGT T GCGGCCGCCTGCAG-- 3'--CTTAAG CGCCGGCG A AGATCT * -------------- C TGATCA A CGCCGGCGGACGTC-- Prefix |
Source
The Recombination Enhancer sequence from Salmonella typhimurium.
References
- Johnson RC and Bruist MF. Intermediates in Hin-mediated DNA inversion: a role for Fis and the recombinational enhancer in the strand exchange reaction. EMBO J 1989 May; 8(5) 1581-90. pmid:2548848.
- Haykinson MJ and Johnson RC. DNA looping and the helical repeat in vitro and in vivo: effect of HU protein and enhancer location on Hin invertasome assembly. EMBO J 1993 Jun; 12(6) 2503-12. pmid:8508775.
- Perkins-Balding D, Dias DP, and Glasgow AC. Location, degree, and direction of DNA bending associated with the Hin recombinational enhancer sequence and Fis-enhancer complex. J Bacteriol 1997 Aug; 179(15) 4747-53. pmid:9244261.
