Reporter
Part:BBa_J31011:Design
Designed by Karmella Haynes Group: iGEM2006_Davidson (2006-10-29)
From partsregistry.org
RBS-RFP reverse
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 7
Illegal AgeI site found at 119
Design Notes
The primers were designed to add PstI-NotI-SpeI to the left and XbaI-NotI-EcoRI to the right (reversal of standard BioBrick cloning sites) so that this part could be cloned in the reverse orientation. Placement of a promoter (reverse) to the right of this part should convey RFP expression.
Source
BBa_I13507
