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Coding
tetA(C)f
Part:BBa_J31007:Design
Designed by Sabriya Rosemond, Erin Zwack Group: iGEM2006_Davidson (2006-07-12)
From partsregistry.org
tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 290
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 316
Illegal NgoMIV site found at 684
Illegal NgoMIV site found at 844
Design Notes
This sequence was amplified from pSB1AT3 and is identical to the Tet resistance gene found in pBR322. BBa_J31007 was cloned into the pSB1A2 plamsid.
This part was PCR amplified from pSB1AT3 using the following primers. The primers have non-annealing 5'- extensions that introduce an XbaI site to the left and a SpeI site to the right of the coding region. Primer annealing sites are shown in bold.
Forward: 5’ GCATTCTAG ATGAAATCTAACAATGCGCTCATC
Reverse: 5’ ATGCACTAG TTAGGTCGAGGTGGCCCGGC
