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DNA Available
Experience: Works
Part:BBa_J23018
Entered: 2006-08-03
Designed by John Anderson Group: iGEM2006_Berkeley
From partsregistry.org
EN-Ptet-rbs-X-[RFP]-SNP "ORF Expresser"
Variant of pBca1021 (J61003) with GFP inserted. In practice, it is a E0040 basic part just in a different pSB1A* plasmid. Part J23019 is the same as J23018 but in pSB3C6.
- RS 16:08, 12 September 2008 (UTC): Note that this plasmid has been split into a plasmid backbone and part. The plasmid backbone is this part (BBa_J23018) and the part is BBa_E1010. The physical DNA associated with this part has sequence BBa_J23018-E1010.
Sequence and Features
EcoRI site found in the sequence at base pair 2058.
XbaI site found in the sequence at base pair 2292.
SpeI site found in the sequence at base pair 2.
PstI site found in the sequence at base pair 16.
NotI site found in the sequence at base pair 9.
NotI site found in the sequence at base pair 2064.
XbaI site found in the sequence at base pair 2292.
SpeI site found in the sequence at base pair 2.
PstI site found in the sequence at base pair 16.
NotI site found in the sequence at base pair 9.
NotI site found in the sequence at base pair 2064.
