Translational_Unit
Part:BBa_J18935
Designed by Raik Gruenberg Group: Affiliates (2010-01-26)
From partsregistry.org
FKBP ~ mCitrine
P03 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture:
- T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop
Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts.
Expression
Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity.
Reference
- Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
[edit]
Categories
//cds
//proteindomain/binding
//proteindomain/binding
Reviews
Parameters
Unrated Part
Experience:
5
(77)
4
(21)
3
(44)
2
(13)
1
(5)
| None |
