Part:BBa_J18902
From partsregistry.org
pSB1AK3F construction plasmid for protein fusion BioBricks
pSB1AK3F is identical to pSB1AK3 but uses the "Freiburg" extension of the standard BioBrick prefix and suffix. This Freiburg extension adds a new pair of inner restriction enzymes -- NgoMIV (prefix) and AgeI (suffix) -- which allows the in-frame fusion of protein parts. See BB formats.
pSB1AK3F is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). For the version of this plasmid with a CcdB insert, see BBa_P1010.
The given sequence starts with the Freiburg BB suffix and ends with the Freiburg BB prefix. The complete vector sequence is therefore obtained from: part + vector
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 3177
Illegal suffix found in sequence at 10
Illegal XbaI site found at 3192 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3177
Illegal XhoI site found at 1044
Illegal XhoI site found at 2070 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 3177
Illegal suffix found in sequence at 11 - 25INCOMPATIBLE WITH RFC[25]Illegal suffix found in sequence at 1
Illegal EcoRI site found at 3177
Illegal XbaI site found at 3192
Illegal NgoMIV site found at 3200
//standard/25
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