DNA Available
Experience: None
Get This Part
Reporter
mCherry

Part:BBa_J06505:Design

Designed by ytwang   Group: iGEM_Harvard   (2005-07-18)

From partsregistry.org


monomeric RFP optimized for bacteria

Content-type: text/html

Software error:

Can't call method "sequence" without a package or object reference at /web-site/partsregistry.org/cgi/lib/AssemblySystem.pm line 216.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.


Design Notes

Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends with the LVA tag using PCR.


Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.



Source

mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible and with an LVA tag added.

References