Plasmid
Part:BBa_I714038:Design
Designed by Anting Xu Group: iGEM07_Peking (2007-10-26)
From partsregistry.org
pSC101 with relaxase and oriT deleted
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 4027
Illegal PstI site found at 2936 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 375
Illegal XhoI site found at 7013 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 4027
Illegal PstI site found at 2936 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 4027
Illegal PstI site found at 2936
Illegal NgoMIV site found at 401
Illegal NgoMIV site found at 769
Illegal NgoMIV site found at 929
Illegal NgoMIV site found at 1283
Illegal NgoMIV site found at 5479
Illegal NgoMIV site found at 7568
Illegal AgeI site found at 1687
Design Notes
Region of deletion: 2936bp - 4115bp (containing mobA and oriT) and substituted with PstI restriction site CTGCAG
Method of deletion: in vitro reversed cyclic PCR and self-ligation.
Source
plasmid pSC101. Genebank NC_002056.
