DNA Planning
Experience: Fails
Get This Part
Plasmid

Part:BBa_I51000:Design

Designed by Reshma Shetty   Group: Knight Lab, MIT   (2006-03-13)

From partsregistry.org

BioBrick base vector, as originally designed

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1327
    Illegal suffix found in sequence at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1327
    Illegal BglII site found at 1783
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1327
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1327
    Illegal XbaI site found at 1342
    Illegal SpeI site found at 2
    Illegal PstI site found at 16


Design Notes

BBa_I51000 is the original design of the BioBrick base vector BBa_I51020. Please see BBa_I51020 for complete design notes on the BioBrick base vector.