Plasmid
Part:BBa_I51000
Designed by Reshma Shetty Group: Knight Lab, MIT (2006-03-13)
From partsregistry.org
BioBrick base vector, as originally designed
BBa_I51000 is the original design of the BioBrick base vector BBa_I51020.
Synthesis of BBa_I51000 was problematic. We encountered two types of issues while trying to construct BBa_I51000. Faulty part design adversely impacted the synthesis process and problems during synthesis ended up compromising the design of our parts. Our synthesis difficulties largely stemmed from the dependence of commercial DNA synthesis processes on cloning, assembly and propagation of synthesized DNA in E. coli.
The BioBrick base vector that was successfully constructed is BBa_I51020.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1327
Illegal suffix found in sequence at 1 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1327
Illegal BglII site found at 1783 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1327
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1327
Illegal XbaI site found at 1342
Illegal SpeI site found at 2
Illegal PstI site found at 16
[edit]
Categories
//plasmid/construction
//plasmidbackbone/version/5
//standard/10
//plasmidbackbone/version/5
//standard/10
Reviews
Parameters
Unrated Part
Experience: Fails
5
(77)
4
(21)
3
(44)
2
(13)
1
(5)
| None |
