DNA
pSC101
Part:BBa_I50040:Design
Designed by Reshma Shetty Group: Knight Lab, MIT (2006-02-03)
From partsregistry.org
nonfunctional pSC101 origin of replication
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal prefix found in sequence at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
- 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Design Notes
Part:pSB4A3 served as the basis for the design of I50040. Two mutations were introduced in order to remove two MfeI sites. These two mutations occur within an inverted repeat and one also occurs within a ribosome binding site. Additional mutations were included to eliminate other potentially useful restriction sites. Thus, the sequence of I50040 differs slightly from that used in Part:pSB4A3. One or more of these mutations rendered I50040 nonfunctional as a replication origin.
Source
This part was synthesized via direct DNA synthesis by Codon Devices, Inc. in Cambridge, MA.
References
- Cohen SN and Chang AC. Revised interpretation of the origin of the pSC101 plasmid. J Bacteriol 1977 Nov; 132(2) 734-7. pmid:334752.
- Lutz R and Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res 1997 Mar 15; 25(6) 1203-10. pmid:9092630.
- Elowitz MB and Leibler S. A synthetic oscillatory network of transcriptional regulators. Nature 2000 Jan 20; 403(6767) 335-8. doi:10.1038/35002125 pmid:10659856.
