DNA Planning
Experience: Works
Get This Part
DNA
p15A

Part:BBa_I50032

Designed by Reshma Shetty   Group: Knight Lab   (2007-04-11)

From partsregistry.org

p15A replication origin

BBa_I50032 is a p15A origin of replication. BBa_I50032 is the replication origin found in the pSB3* series of BioBrick vectors.

Usage and Biology

  • BBa_I50032 yields vectors with an expected copy number of 10-12 per cell (Molecular Cloning).

References

Engineering BioBrick vectors from BioBrick parts
Journal of Biological Engineering, 2008 Apr 14;2:5
Reshma Shetty, Drew Endy, Tom Knight
URL
GenBank EU496097

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 811
    Illegal AgeI site found at 1134


[edit]
Categories
//plasmid/component/origin
Reviews
Unrated Part
Experience: Works
5
(77)
4
(21)
3
(44)
2
(13)
1
(5)
Parameters
None